This paper analyzes the phenomenon of differentiation of netizens and its reason by using literature analysis methods. It is analyzed that the diversity of information needs drives the dissemination of public opinion of the netizens, and the negative impact of information alienation changes the direction of the dissemination of the differentiation of public opinion among netizens. Social trust blocks the dissemination of positive public opinion and causes netizens to divide. Analysis of the evolution law after the formation of differentiation of netizens showed: the focus, diffusion, and elimination of rumors in the process of the dissemination of Internet public opinion were all in line with the SEIR mechanism of the system dynamics. Therefore, Differentiating and differentiating Internet users transformation has many possible paths. It is proposed that the government should correctly identify and protect the reasonable interests of Internet users, scientifically adopt intervention measures for Internet public opinion, rationally control the monitoring costs and costs of Internet public opinion communication, and correctly select the guiding methods for Internet public opinion communication, and make full use of the law of differentiation to guide the control of network public opinion.