As the most important scientific research support resource, the Science Funding is worthy of attention in the role and status of "Double First-class" construction. This paper takes 68 natural subjects in the "Double First-class" construction subjects as the research object and uses 521,938 research papers and related citations of the Web of Science database from 2012 to 2016 to examine the science fund's "Double First-class" construction subject in terms of pre-funding status, output situation and impact. The results show that in the period of "Double First-class" construction of 2012-2016, the science fund has funded the "Double First-class" construction subjects precisely, and the accuracy reflects in the two aspects of funding ratio and influence. Among the 68 "Double First-class" construction subjects, the funding ratio of 52 of them is higher than that of the average level of China, and the influence of the papers in 58 subjects has improved.